Lattice SIM 3 Launch Tour in Spain

Get ready for this exclusive invitation to the ZEISS Lattice SIM 3 launch tour.

Fast Optical Sectioning of Developing Organisms and Tissue Microstructures

ZEISS Lattice SIM 3 is specifically designed to meet the imaging requirements of multicellular organisms and tissue sections. This system exploits the full potential of the SIM Apotome technology: fast optical sectioning at superior quality, large fields of view with access to smaller regions of interest, near-isotropic resolution, and the gentlest super-resolution imaging possible.

Capture entire model organisms and tissue sections
Acquire super-resolution images as fast and gentle as widefield images
Go from a large-field overview to the super-resolution details

Capture entire model organisms and tissue sections

ZEISS Lattice SIM 3 is your system of choice for fast imaging of larger volumes, such as 3D model organisms, embryos, organoids, or tissue sections. Whether you work with living or fixed samples, ZEISS Lattice SIM 3 provides access to structured illumination microscopy of multicellular organisms with superior penetration depth.

Caption: Spheroid stained for mitochondria (MitoTracker Green) and nuclei (NucRed Live 647).

Acquire super-resolution images as fast and gentle as widefield images

Choose between the standard SIM Apotome imaging mode for the highest available resolution or the imaging mode with reduced phases for slightly lower resolution but significantly increased speed and gentleness. Combine SIM Apotome with the Leap mode to significantly speed up super-resolution acquisition.

Caption: Spheroid invading collagen matrix; cells are expressing Lifeact-tdTomato; color-coded depth projection.

Go from a large-field overview to the super-resolution details

For large sample experiments, ZEISS Lattice SIM 3 offers the most advantageous combination of a large field of view and super-resolution imaging. SIM Apotome mode in combination with SIM² image reconstruction enables lateral super-resolution down to 140 nm with superior optical sectioning and sensitivity. Additionally, imaging in Lattice SIM mode with a ZEISS 25× multi-immersion objective and subsequent SIM² processing provides similar lateral resolutions with larger fields of view and more flexible adaptation to the refractive index of your sample.

Caption: Murine brain imaged in SIM Apotome and Lattice SIM modes over a Z stack range of 170 µm. Volume rendering: LD LCI Plan-Apochromat 25× / 0.8 Imm Corr. Sample courtesy of Herms Lab (MCN, University of Munich, Germany).

AGENDA

April 29th Online introduction and demonstration
10:00-10:30 Online introduction to the Lattice SIM 3
10:30-11:30 Online demonstration of the Lattice SIM 3

Presenter Dr. Claudia Kästner

Claudia Kästner is an experienced Application Specialist at ZEISS Microscopy, where she is responsible for providing tailored demonstrations of super-resolution microscopy solutions using structured illumination (SIM). Prior to joining ZEISS in 2021, Claudia worked as a Research Specialist in the Department of Molecular Biology at Princeton University. With a PhD from Freie Universität Berlin, Claudia has a strong background in Chemistry and brings a deep understanding of advanced microscopy techniques to her role.

April 29th-30th Onsite demonstration in Barcelona
Carl ZEISS Iberia in collaboration with the Centre for Genomic Regulation (CRG), invite you to participate in the onsite demonstration of the Lattice SIM 3 system that will be held in the Advance Light Microscopy Unit (ALMU) at the CRG.

May 8th-9th Onsite demonstration in Bilbao
Carl ZEISS Iberia in collaboration with the UPV, invite you to participate in the onsite demonstration of the Lattice SIM 3 system that will be held in SKIGER. Campus de Leioa.

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