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Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming. Courtesy of Jonathan Gabriel Piccirillo
Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming. Courtesy of Jonathan Gabriel Piccirillo

Correlative Cryo Workflow Workshop

19th and 20th June 2024

 

At Centro Nacional de Biotecnología (CNB), Campus Cantoblanco, Madrid

Come join us for an exciting workshop where you can discover the process of image formation, sample preparation, and learn about the different types of cryo confocal and focused ion beam scanning electron microscopes - all under the guidance of experts in the field.
This workshop intends to be a truly immersive experience, so we're providing hands-on access to the latest Cryo CLEM technology and 3D software processing solutions from ZEISS.
Join us in this 2-day workshop and take your knowledge in microscopy to the next level.
 



Register now

Centro Nacional de Biotecnología, Campus Cantoblanco, Madrid

In collaboration with Centro Nacional de Biotecnología and Servicio de Criomicroscopía'

Dra. Rocío Arranz
Dr. Javier Conesa
Dr. Francisco Javier Chichón

In collaboration with Centro Nacional de Biotecnología

Correlative Cryo Workflow Workshop

This workshop will cover methods and supporting theory to enable attendees to become more comfortable and competent operating the microscopes for cryo correlative confocal light electron microscopy (cryo CLEM) workflow and image processing/analysis. The CNB-CSIC will host this workshop in collaboration with ZEISS in Madrid.

This is a two days in-person free workshop consisting of presentations to highlight:

  • Fundamental microscopy concepts of the cryo clem workflow

  • hands-on instrument time with trainers to click through your workflows step by step

  • With small group sizes of <5 attendees/trainer, you will get valuable, individualized access to the most cutting-edge cryo correlative microscopy solutions ZEISS offers.

  • 09:30 - 10:00

    Registration

    10:00 - 10:15

    Welcome

    Prof. José María Valpuesta, CNB

    10:15 - 11:00

    CNB Presentation

    Dr. Javier Conesa, CNB

    11:00 - 11:45

    Correlative Cryo Workflow

    Dr. Abel Sainz, ZEISS

    11:45 - 12:00

    Coffee Break

    12:00 - 12:45

    Correlative Microscopy Software Solutions

    Dr. Dieter Lauer, ZEISS

    12:45 - 13:30

    Dissecting the lifecycle of uropathogens by directly analyzing patient samples with cryo-electron tomography

    Dr. Gregor Weiss, ETH Zürich, Institute of Molecular Biology & Biophysics

    13:30 - 14:30

    Lunch, finger food

    14:30 - 16:00

    Hands-on sessions:

    •  Cryo FIB-SEM session 1
    •  Cryo Confocal session 1
    •  Software processing session 1

     

    • Dr. Andreas Schertel
    • Dra. Mirjam Geibel
    • Dr. Dieter Lauer

  • 09:00 - 10:30

    Practical paralell session

    • Cryo FIB-SEM session 2
    • Cryo Confocal session 2
    • Software processing session 2
     
    • Dr. Andreas Schertel
    • Dra. Mirjam Geibel
    • Dr. Dieter Lauer

    10:30 - 12:00

    Practical paralell session

    • Cryo FIB-SEM session 3
    • Cryo Confocal session 3
    • Software processing session 3

     

    • Dr. Andreas Schertel
    • Dra. Mirjam Geibel
    • Dr. Dieter Lauer

    12:00 - 13:00

    Round Table Discussion 

    All

    13:00 - 13:15

    Closing and remarks

    Dr. David Jiménez, ZEISS

Live Demonstration

Experience the following electron microscope systems in small groups and discuss directly with the experts

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